Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid procedure for detecting recombinant plasmids using butanol extraction.

Y M Mak1, R Sornarajah, K K Ho

  • 1Botany Department, National University of Singapore, Republic of Singapore.

Biotechniques
|December 1, 1991
PubMed
Summary

Researchers developed a simple butanol extraction method for bacterial DNA. This technique allows for quick screening of bacterial cultures for recombinant plasmid DNA without costly purification steps.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiological progression of end colostomy trephine diameter and area.

BJS open·2019
Same author

Pleuroscopy: our initial experience in Hong Kong.

Hong Kong medical journal = Xianggang yi xue za zhi·2008
Same author

Many forks in the path: cycling with FoxO.

Oncogene·2008
Same author

The short-to-midterm results of endovascular stent grafting for acute thoracic aortic diseases in Chinese patients.

Hong Kong medical journal = Xianggang yi xue za zhi·2006
Same author

Influence of demographic factors and sport type on growth hormone-responsive markers in elite athletes.

The Journal of clinical endocrinology and metabolism·2006
Same author

Erythropoietin administration does not influence the GH--IGF axis or makers of bone turnover in recreational athletes.

Clinical endocrinology·2005

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacterial DNA extraction is crucial for genetic analysis.
  • Current methods can be time-consuming and require expensive reagents.
  • Efficient screening for recombinant plasmid DNA is essential in molecular biology.

Purpose of the Study:

  • To describe a novel, convenient DNA extraction procedure for bacteria.
  • To evaluate the utility of butanol for bacterial DNA isolation.
  • To enable rapid screening of bacterial cultures for recombinant plasmid DNA.

Main Methods:

  • Utilized butanol as the primary solvent for DNA extraction from bacterial cultures.
  • Developed a simplified protocol minimizing purification steps.
  • Focused on applicability to screening for recombinant plasmid DNA.

Related Experiment Videos

Main Results:

  • A straightforward and rapid DNA extraction method was established.
  • The procedure effectively isolates bacterial DNA suitable for screening.
  • Eliminated the need for extensive purification and expensive chemicals.

Conclusions:

  • Butanol-based DNA extraction offers a convenient and cost-effective alternative.
  • This method is highly suitable for high-throughput screening of bacterial recombinant plasmid DNA.
  • Simplifies molecular biology workflows in bacterial research.