Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An extended dynamical hydration shell around proteins.

Simon Ebbinghaus1, Seung Joong Kim, Matthias Heyden

  • 1Lehrstuhl für Physikalische Chemie II, Ruhr-Universität Bochum 44780 Bochum, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|December 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Light-induced quantum friction of carbon nanotubes in water.

Nature·2026
Same author

Ion-Pair Specific Response of Water to Electric Fields.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

THz spectroscopic fingerprints of the hydration upon globular protein assembly.

The Journal of chemical physics·2026
Same author

Identifying structural and dynamic features of proteins for machine learning models that predict rates of energy transfer.

The Journal of chemical physics·2026
Same author

A Film of Hydrophobic Cations Reshapes the Electric Double Layer at the Metal/Water Interface.

Journal of the American Chemical Society·2026
Same author

Fast sampling of protein conformational dynamics.

Science advances·2026
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Hydration water dynamics significantly impact protein folding. Terahertz spectroscopy reveals that protein solvation layers extend beyond 20 angstroms, influencing water network motion.

Area of Science:

  • Biophysics
  • Physical Chemistry
  • Structural Biology

Background:

  • Protein folding research traditionally emphasizes protein structure, neglecting the significant role of hydration water.
  • The interplay between hydration dynamics and protein dynamics is crucial for understanding protein folding.
  • Key questions remain about the extent of biomolecule influence on surrounding water and how protein proximity affects hydration properties.

Purpose of the Study:

  • To investigate the dynamics of solvation layers around proteins using terahertz spectroscopy.
  • To determine the width of the dynamical hydration layer and its influence on protein folding.
  • To explore the concentration-dependent effects on solvation dynamics and water network motion.

Main Methods:

  • Terahertz (THz) spectroscopy to probe solvation dynamics directly.

Related Experiment Videos

  • Measurement of THz absorbance as a function of protein:water molar ratio for a five-helix bundle protein (lambda(6-85)*).
  • Molecular dynamics simulations to analyze water dynamics and compare with experimental results.

    • Main Results:

    • Terahertz spectroscopy directly measures solvation dynamics and the width of the hydration layer.
    • An unexpected nonmonotonic trend in THz absorbance was observed with varying protein concentration.
    • Molecular dynamics simulations showed distinct water dynamics within ~10 Å of proteins, with overlapping layers affecting water motion beyond 20 Å.

    Conclusions:

    • Hydration water plays a critical role in protein structure and energy, comparable to protein components.
    • Solvation layers around proteins extend significantly, influencing the correlated motion of the water network.
    • The study demonstrates THz spectroscopy as a powerful tool for characterizing protein hydration dynamics and their impact on protein folding.