Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
Microbes in Food Production01:29

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Fermentation01:29

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of rumen microbiota in lactating Holstein cows fed molasses versus corn grain at two levels of rumen-degradable protein.

Frontiers in microbiomes·2026
Same author

Comparison of 2-pool and 3-pool digestion kinetic model predictions of neutral detergent fiber digestibility of forages from commercially available data.

Journal of dairy science·2024
Same author

Methane emission, nutrient digestibility, and rumen microbiota in Holstein heifers fed 14 different grass or clover silages as the sole feed.

Journal of dairy science·2023
Same author

Invited review: Corrected milk: Reconsideration of common equations and milk energy estimates.

Journal of dairy science·2023
Same author

Substitution of cane molasses for corn grain at two levels of degradable protein. I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.

Journal of dairy science·2022
Same author

Substitution of molasses for corn grain at two levels of degradable protein. II. Effects on ruminal fermentation, digestion, and nitrogen metabolism.

Journal of dairy science·2022

Related Experiment Video

Updated: Jul 9, 2026

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

In vitro fermentation vessel type and method alter fiber digestibility estimates.

M B Hall1, D R Mertens

  • 1US Dairy Forage Research Center, USDA-ARS, Madison, WI 53706, USA. mbhall@wisc.edu

Journal of Dairy Science
|December 22, 2007
PubMed
Summary

Fermentation vessel type significantly impacts neutral detergent fiber digestibility (NDFD) measurements. Polyethylene tubes with gas-release valves yielded the lowest NDFD, affecting net energy calculations and highlighting the need for method standardization in feed analysis.

More Related Videos

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
06:58

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

Related Experiment Videos

Last Updated: Jul 9, 2026

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
07:15

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

Published on: July 31, 2019

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
06:58

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
06:23

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Published on: February 15, 2019

Area of Science:

  • Animal and Nutritional Sciences
  • Rumen Microbiology
  • Forage Quality Analysis

Background:

  • Accurate in vitro determination of neutral detergent fiber digestibility (NDFD) is crucial for predicting forage quality and animal nutrition.
  • Variations in experimental setups, including vessel type and gas management, can influence NDFD results.
  • Standardization of in vitro fermentation techniques is essential for reliable and comparable data across studies.

Purpose of the Study:

  • To compare the effects of different vessel types and fermentation conditions on in vitro neutral detergent fiber digestibility (NDFD) measurements.
  • To evaluate the impact of these variations on the prediction of net energy for lactation (NEL).
  • To determine if NDFD results are comparable across different in vitro fermentation methods and substrates.

Main Methods:

  • Six treatments involving 50-mL and 125-mL vessels (polyethylene tubes, Erlenmeyer flasks, serum vials) with varying gas release and gassing methods were employed.
  • In vitro fermentations were conducted for 48 hours using standard medium and ruminal inoculum with substrates including alfalfa hay, corn silage, ryegrass hay, and soyhulls.
  • Neutral detergent fiber (NDF) analysis was performed at multiple time points to calculate NDFD, and gas production was monitored.

Main Results:

  • The lowest NDFD values were consistently observed with polyethylene centrifuge tubes featuring gas-release valves (Treatment 1).
  • Treatments involving sealed vessels with manual gas release or continuous gassing (Treatments 3, 4, 5, 6) yielded higher and comparable NDFD values.
  • Differences in NDFD among treatments varied with substrate type, with soyhulls showing the greatest variation and alfalfa the least.
  • Predicted Net Energy for Lactation (NEL) values were 7-15% lower for Treatment 1 compared to other methods.

Conclusions:

  • Measured NDFD is significantly altered by the choice of fermentation vessel and gas management technique.
  • Polyethylene tubes with gas-release valves lead to an underestimation of NDFD, impacting nutritional value predictions.
  • NDFD values are not directly comparable across different in vitro fermentation methods, and the interaction between method and substrate type is important.