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Rapid method for the determination of ketanserin in rat serum by high-performance liquid chromatography with
1Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284.
Journal of Chromatography
|November 15, 1991
Abstract:
A simplified high-performance liquid chromatographic (HPLC) assay for the determination of ketanserin in rat serum is described. The chromatographic method allowed complete resolution of ketanserin from two of its metabolites. A protein precipitation extraction procedure was employed which allowed rapid sample preparation for injection into the HPLC system. Both intra- and inter-assay coefficients of variation at serum ketanserin concentration of 200 and 800 ng/ml were less than 6% and the accuracy was excellent. The assay has been applied for determining the elimination kinetics of ketanserin in the rat.