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Direct sequencing of double-stranded polymerase chain reaction-amplified 16S rDNA
B Both1, G Krupp, E Stackebrandt
1Institute for General Microbiology, University of Kiel, Federal Republic of Germany.
Analytical Biochemistry
|December 1, 1991
Abstract:
A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing.