[Galactosyl neoglycoalbumin:preparation and studies of its binding with hepatic special receptor]

Galactosyl-neoglycoalbumin (NGA) can bind with the asialoglycoproteins receptor specifically on the surface of mammal's liver. We prepared NGA in different carbohydrate density and made some animal and clinical tests with technetium-99m labeled NGA. The maximum absorption of 99mTc-NGA-21 was 82.6% in liver. We also got the satisfactory radiographs of liver. NGA was synthesized by the covalent coupling of a carbohydrate bifunctional reagent, 2-imino-2-methoxyethyl-1-thio-galactose (4), to human serum albumin. The cyanomethyl-1-thio-glycoside of beta-D-galactose (3) was prepared from the pseudothiourea derivative (2) which was synthesized by acetylation, bromination and substitution of galactose (3) could be converted to (4) by treatment with sodium methoxide.