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Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
[A contribution to the nomenclature and classification of chlamydiae]
Abstract:
The results of molecular-genetic study of typical specimens of the genus Chlamydophila of the family Chlamydiaceae from the FSE FCTRSA collection of microorganisms are considered for the purpose of their taxonomy based on comparative analysis by the omp1-, omp2-, 16S rRNA-, and 23S rRNA-genes and by the presence of extrachromosomal plasmid with corresponding fragments of the genomes officially registered species of chlamydiae. According to the omp1-RFLP-AluI-profile, the Rostinovo-70, 250, PP-87, and KC-93 strains were characterized by new genotype of chlamydiae (genotype G). The new omp2-RFLP-AluI-profile of chlamydiae on carrying out the comparative analysis of Rostinovo-70, 250, PP-87 and KC-93 strains by omp2-gene was characterized. On the basis of the species identification of chlamydiae by genetical and ecological criteria, the chlamydia strains Rostinovo-70, 250, PP-87, and KC-93 can be included in the new chlamydia species Chlamydophila parapsittaci sp. nov. It was suggested to combine into a new species the following strains of Chlamydophila psittaci: WC, NJ1, 92-1293, TT3, 7344/2, GD, CT1, Par1, 84/2334, R54, VS225, 777B15, and Prk/Daruma.
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