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Related Experiment Videos

High affinity selenium uptake in a keratinocyte model.

Dennis Ganyc1, William T Self

  • 1Department of Molecular Biology and Microbiology, Burnett College of Biomedical Science, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816-2364, USA.

FEBS Letters
|December 25, 2007
PubMed
Summary
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Selenium is distributed in mammals primarily via selenoprotein P. Researchers found that hydrogen selenide is actively transported into human cells, suggesting it

Area of Science:

  • Biochemistry
  • Cell Biology
  • Trace Element Metabolism

Background:

  • Selenium distribution in mammals is mainly mediated by selenoprotein P.
  • Selenium reaches organs and tissues even without selenoprotein P when supplied in the diet.
  • The specific form of selenium actively taken up by mammalian cells at trace concentrations remains undetermined.

Purpose of the Study:

  • To investigate whether the reduction of selenite to selenide affects selenium uptake in mammalian cells.
  • To identify the actively transported form of selenium in human keratinocytes.

Main Methods:

  • Utilized a human keratinocyte cell model.
  • Assessed selenium uptake by varying selenite concentrations and observing reduction to selenide.
  • Investigated the kinetics of uptake, including apparent K(m).

Related Experiment Videos

  • Examined the effect of ATPase inhibitors, molybdate, chromate, and sulfate on selenide uptake.

    • Main Results:

    • A reduced form of selenium, presumed to be selenide (HSe-), was actively transported into keratinocytes.
    • Selenide uptake exhibited saturation kinetics with an apparent K(m) of 279 nM.
    • Uptake was inhibited by ATPase inhibitors and competing anions molybdate and chromate, but not sulfate.

    Conclusions:

    • Hydrogen selenide (HSe-) is likely the small molecule form of selenium actively distributed in mammalian tissues.
    • This active transport mechanism highlights a key pathway for cellular selenium acquisition.
    • Despite its reactivity and oxygen sensitivity, hydrogen selenide plays a crucial role in selenium distribution.