Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent protein applications in plants.

R Howard Berg1, Roger N Beachy

  • 1Integrated Microscopy Facility, Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.

Methods in Cell Biology
|December 25, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunolocalization and Ultrastructure Show Ingestion of Cry Protein Expressed in <i>Glycine max</i> by <i>Heterodera glycines</i> and Its Mode of Action.

Molecular plant-microbe interactions : MPMI·2024
Same author

US-China science collaboration to solve global grand challenges.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Extensive membrane systems at the host-arbuscular mycorrhizal fungus interface.

Nature plants·2019
Same author

Distribution of TMV movement protein in single living protoplasts immobilized in agarose.

The Plant journal : for cell and molecular biology·2018
Same author

A novel bi-domain plant defensin MtDef5 with potent broad-spectrum antifungal activity binds to multiple phospholipids and forms oligomers.

Scientific reports·2017
Same author

Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll f.

Photosynthesis research·2017
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Fluorescent proteins (FPs) are revolutionizing plant cell biology imaging. Advanced microscopy and spectral imaging techniques enable detailed visualization of molecular dynamics and interactions within plant cells.

Area of Science:

  • Plant Cell Biology
  • Molecular Imaging
  • Biophysics

Background:

  • Fluorescent proteins (FPs) are essential tools in plant cell biology.
  • Established genetic techniques facilitate FP-tagged molecule imaging.
  • Advanced microscopy techniques like confocal and multiphoton microscopy are routinely used.

Purpose of the Study:

  • To highlight the significant contributions of FPs to plant cell biology.
  • To discuss the methodologies employed for FP imaging in plants.
  • To explore the applications and future potential of FPs in plant science.

Main Methods:

  • Transient expression and Agrobacterium-mediated transformation for FP-tagged constructs.
  • Confocal and multiphoton microscopy for high-resolution imaging.

Related Experiment Videos

  • Spectral imaging to resolve overlapping FP and autofluorescence signals.
  • Targeting FPs to various organelles and cellular compartments.

    • Main Results:

    • FPs can be localized to most plant organelles, including the nucleus and vacuoles.
    • Spectral imaging effectively separates signals from different FPs and plant autofluorescence.
    • FP-tagged proteins have revealed dynamic processes like membrane trafficking and inclusion body formation.
    • Advanced techniques like Fluorescence Lifetime Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS) enable quantitative analysis.

    Conclusions:

    • Fluorescent proteins are indispensable for studying plant cell dynamics and molecular interactions.
    • Ongoing advancements in imaging technologies promise a bright future for FP applications in plant science.
    • FPs provide critical insights into protein localization, trafficking, and molecular behavior in plant cells.