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Electrochemical sensor with flavin-containing monooxygenase for triethylamine solution.
Hirokazu Saito1, Takeshi Shirai, Hiroyuki Kudo
1Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan.
A novel bioelectronic sensor utilizing flavin-containing monooxygenase type 3 (FMO-3) effectively detects triethylamine (TEA). This biosensor demonstrates good reproducibility and potential for improved selectivity in detecting nitrogen and sulfur compounds.
Area of Science:
- Bioelectronics
- Biosensor development
- Enzyme immobilization
Background:
- Triethylamine (TEA) detection is crucial in various industrial and environmental applications.
- Existing methods for TEA detection may lack sensitivity or selectivity.
- Flavin-containing monooxygenases (FMOs) offer potential for specific enzymatic detection.
Purpose of the Study:
- To develop a novel bioelectronic sensor for sensitive and selective detection of triethylamine (TEA).
- To optimize the biosensor performance by investigating pH effects and employing a substrate regeneration system.
- To explore the potential of different FMO isomers for enhanced selectivity towards nitrogen and sulfur compounds.
Main Methods:
- Development of a Clark-type dissolved-oxygen electrode-based biosensor with immobilized flavin-containing monooxygenase type 3 (FMO-3).
- Enzyme immobilization using poly(vinyl alcohol) containing stilbazolium groups (PVA-SbQ) and photo-irradiation.
- Implementation of a substrate regeneration cycle using L-ascorbic acid (AsA) to amplify biosensor output.
- Systematic study of pH effects on TEA determination, with optimal performance at pH >9.0.
- Construction and testing of a measurement system integrating the FMO-3 biosensor with potentiostat and computer interface.
- Evaluation of biosensor reproducibility and stability over time.
- Fabrication and testing of biosensors using FMO isomers (1, 3, and 5) to assess selectivity for nitrogen and sulfur compounds.
Main Results:
- The developed FMO-3 biosensor successfully measured TEA in the range of 0.5 to 4.0 mmol L(-1).
- The biosensor exhibited good reproducibility with a 6.31% coefficient of variation for five measurements.
- The output current remained stable for several hours, indicating good operational stability.
- Optimal TEA determination was achieved at pH >9.0.
- Biosensors constructed with different FMO isomers showed distinct output patterns for nitrogen and sulfur compounds, suggesting improved selectivity.
Conclusions:
- A robust bioelectronic sensor for TEA detection based on FMO-3 has been successfully developed.
- The substrate regeneration cycle with L-ascorbic acid effectively amplifies the biosensor signal.
- The study demonstrates the potential for enhanced selectivity and the possibility of detecting various nitrogen and sulfur compounds by utilizing different FMO isomers.
