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Characterizing host immune responses by monitoring fluorescently marked cells

Axel Mischo1, Cheryl Eppolito, Qingsheng Li

  • 1Department of Immunology, Roswell Park Cancer Institute, Buffalo, New York, USA.

Immunological Investigations
|December 29, 2007
PubMed
Abstract

No abstract available in PubMed .

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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