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Published on: October 4, 2024
Adhesion to chondroitinase ABC treated dentin
Annalisa Mazzoni1, David H Pashley, Alessandra Ruggeri
1Department of SAU and FAL, University of Bologna, Bologna, Italy.
Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|December 29, 2007
Summary
Removing chondroitin sulphate from dentin enhances resin bonding. Enzymatic treatment of dentin with chondroitinase ABC significantly improved microtensile bond strength and reduced nanoleakage for dental adhesives.
Area of Science:
- Biomaterials Science
- Dental Adhesion
- Biochemistry
Background:
- Dentin bonding requires complete resin infiltration of the demineralized collagen matrix.
- Chondroitin sulphate-glycosaminoglycans influence dentin's structure and hydrophilicity.
Purpose of the Study:
- To evaluate the effect of chondroitinase ABC treatment on the bond strength of two etch-and-rinse adhesives to dentin.
- To investigate the impact of chondroitin sulphate removal on dentin's interfacial properties.
Main Methods:
- Human molars were treated with chondroitinase ABC.
- Immunohistochemistry and scanning electron microscopy visualized collagen and chondroitin sulphate distribution.
- Microtensile bond strength testing and transmission electron microscopy analyzed nanoleakage.
Main Results:
- Chondroitinase ABC effectively removed chondroitin 4/6 sulphate.
- Both tested adhesives showed increased microtensile bond strength on treated dentin.
- Prime and Bond NT exhibited a 92% increase in bond strength, while Adper Scotchbond Multi-Purpose showed a 28% increase.
Conclusions:
- Removal of chondroitin sulphate and dermatan sulphate enhances dentin adhesion.
- This enhancement is likely due to reduced water content and increased interfibrillar spaces.
- Further research is needed to assess the long-term stability of treated dentin interfaces.
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