[Microbial flora in Cerasus sachalinensis rhizosphere]

Area of Science:

• Microbiology
• Plant Science
• Ecology

Background:

• The rhizosphere microbiome plays a crucial role in plant health and nutrient cycling.
• Understanding plant-associated microbial communities is essential for sustainable agriculture and forestry.
• Cerasus sachalinensis (sachalin cherry) is a significant tree species with potential ecological and economic importance.

Purpose of the Study:

• To isolate, identify, and classify the microbial communities in the Cerasus sachalinensis rhizosphere.
• To investigate the community structure and dynamic changes of these microbes at different growth stages of C. sachalinensis.
• To determine the dominant genera of bacteria, Actinomyces, and fungi in the rhizosphere.

Main Methods:

• Isolation and identification of microbes using selected culture media.
• Classification of isolated bacteria, Actinomyces, and fungi.
• Analysis of microbial community structure and dynamics.
• Correlation of microbial composition with C. sachalinensis growth stages.

Main Results:

• Bacteria from 15 genera were isolated, with Bacillus, Pseudomonas, and Flavobacterium being dominant.
• Flavus and Albosporus were the dominant Actinomyces genera.
• Mucor, Aspergillus, and Penicillium were the main fungal genera.
• Microbial flora richness varied with plant growth stage, peaking at defoliation and lowest at budding.

Conclusions:

• The Cerasus sachalinensis rhizosphere harbors a diverse microbial community comprising bacteria, Actinomyces, and fungi.
• Dominant microbial genera were identified, providing insights into the functional potential of the rhizosphere.
• Microbial community structure and dynamics are significantly influenced by the growth stage of C. sachalinensis.
• The findings highlight the importance of plant growth stage in shaping rhizosphere microbial ecology.