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Imprinting of an evolutionarily conserved antisense transcript gene APeg3.

Jung Ha Choo¹, Jeong Do Kim, Joomyeong Kim

¹Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.

|January 2, 2008

Summary

A novel antisense transcript gene, APeg3, has been identified in the mammalian PEG3 locus. This imprinted gene, expressed from the paternal allele, is conserved across species, suggesting important functions.

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Area of Science:

Genetics
Genomics
Molecular Biology

Background:

The PEG3 locus is known to harbor imprinted genes.
Antisense transcripts are increasingly recognized for their regulatory roles.

Purpose of the Study:

To identify and characterize the APeg3 antisense transcript gene.
To investigate the evolutionary conservation and imprinting status of APeg3.

Main Methods:

Expressed Sequence Tag (EST) database analysis.
5' and 3' Rapid Amplification of cDNA Ends (RACE) experiments.
Strand-specific RNA analysis.

Main Results:

A homologous APeg3 transcript was found in mouse, cow, and human EST databases.
A 900-bp cDNA sequence of mouse APeg3 was identified, located in the 3'UTR of Peg3.
Mouse APeg3 exhibits paternal allele-specific expression, indicating imprinting.
APEG3 expression was confirmed in human and cow adult brains.

Conclusions:

The mammalian PEG3 locus contains an evolutionarily conserved antisense transcript gene, APeg3.
APeg3 displays paternal imprinting in the brain.
The conserved nature of APeg3 suggests functional significance within the imprinted PEG3 domain.