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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

A self-feeding roller bottle for continuous cell culture.

R Eric Berson¹, Goetz Friederichs

¹Department of Chemical Engineering, University of Louisville, Louisville, Kentucky 40292, USA. eric.berson@louisville.edu

Biotechnology Progress

|January 4, 2008

Summary

A novel self-feeding roller bottle continuously supplies fresh media to cell cultures. This innovation enhances antibody production rates significantly compared to traditional methods.

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Area of Science:

Biotechnology
Cell Culture Technology
Bioprocess Engineering

Background:

Conventional roller bottles require manual media exchange, limiting continuous cell culture.
Optimizing cell culture conditions is crucial for maximizing product yield, such as antibodies.

Purpose of the Study:

To introduce a mechanically simple, self-feeding roller bottle design.
To evaluate the performance of this novel roller bottle for continuous cell culture and antibody production.

Main Methods:

A conventional roller bottle was modified with two chambers: one for media reservoir and one for cell culture.
A spiroid of tubing within the media reservoir acted as a pump, driven by bottle rotation.
The system was tested over 10 days using a hybridoma cell line.

Main Results:

The self-feeding roller bottle maintained steady-state cell densities.
Continuous volume dilution resulted in lower densities than batch operation.
Steady-state density was controllable by adjusting perfusion rate via bottle rotation speed.
Specific antibody production rate was up to 3.7 times higher than in conventional roller bottles.

Conclusions:

The self-feeding roller bottle is a viable and mechanically simple system for continuous cell culture.
This technology offers a significant improvement in specific antibody production rates.
The system allows for controllable cell densities through adjustable perfusion rates.