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Updated: Jul 8, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
Published on: October 29, 2015
3-drug synergistic interactions of small molecular inhibitors of hepatitis C virus replication
Christian Grünberger1, David L Wyles, Kelly A Kaihara
1Department of Medicine, Division of Infectious Diseases, University of California, San Diego, La Jolla, USA.
Abstract:
Small molecular inhibitors of hepatitis C virus (HCV) replication provide remarkable potency, but the rapid selection of resistance mutations will require that these agents be used in combination for clinical treatment. Using a model HCV replicon system, we have extended prior in vitro studies of double combinations of candidate small molecular inhibitors to studies evaluating the simultaneous use of 3 agents. This was done in an effort to anticipate conditions that might ultimately be required clinically. We formally demonstrate synergistic antiviral activity with 3-drug combinations in this model, further supporting the concept of clinical investigations of combination therapy for HCV infection.
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