Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variation in the access and use of biologic and targeted synthetic DMARD in juvenile idiopathic arthritis: insights from a national UK survey.

Pediatric rheumatology online journal·2026
Same author

Referral, treatment patterns and change in quality of life in the first twelve months in children and young people with Juvenile Idiopathic Arthritis: an analysis of the association with ethnicity and socioeconomic position using data from a cohort study.

Rheumatology (Oxford, England)·2026
Same author

Holistic management of juvenile idiopathic arthritis across all ages: British Society for Rheumatology Guideline scope.

Rheumatology advances in practice·2026
Same author

Autoantibodies Predictive of Atherosclerosis Progression and Statin Response in Juvenile-Onset SLE: A Biomarker Discovery Study.

medRxiv : the preprint server for health sciences·2026
Same author

(-)-Englerin A binding to human TRPC5 exposes an aromatic interaction network in channel activation.

Nature communications·2026
Same author

Endothelial Senescence Drives Deleterious Endothelial-Adipocyte Cross-Talk in Patients With Heart Failure and Type 2 Diabetes.

JACC. Basic to translational science·2026

Related Experiment Video

Updated: May 11, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

TRPC channel activation by extracellular thioredoxin.

Shang-Zhong Xu1, Piruthivi Sukumar, Fanning Zeng

  • 1Institute of Membrane and Systems Biology, Garstang Building, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.

Nature
|January 4, 2008
PubMed
Summary

Extracellular thioredoxin activates TRPC5 and TRPC1 ion channels by breaking a disulfide bridge. This newly found mechanism links thioredoxin to cell function, particularly in rheumatoid arthritis.

More Related Videos

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

Related Experiment Videos

Last Updated: May 11, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

Area of Science:

  • Ion channel biology
  • Molecular cell biology
  • Rheumatology

Background:

  • Mammalian Transient Receptor Potential (TRP) channels function as cellular sensors.
  • Identifying endogenous activators and cellular functions of TRP channels is a key research goal.
  • Extracellular thioredoxin, a redox protein, has largely unknown extracellular targets.

Purpose of the Study:

  • To investigate the activation mechanism of TRPC5 and TRPC1 channels.
  • To determine the role of extracellular thioredoxin in regulating these channels.
  • To explore the implications for rheumatoid arthritis.

Main Methods:

  • Biochemical assays to study channel activation by thioredoxin.
  • Analysis of TRPC5 and TRPC1 expression in synoviocytes.
  • Functional studies involving channel blockade and secretory activity measurement.

Main Results:

  • Reduced thioredoxin activates TRPC5 homomultimeric and TRPC5-TRPC1 heteromultimeric channels.
  • Activation occurs via disruption of a disulfide bridge in the extracellular loop of TRPC5.
  • TRPC5 and TRPC1 are expressed in rheumatoid arthritis synoviocytes and activated by thioredoxin.
  • Blocking these channels affects cell secretory activity.

Conclusions:

  • A novel ion channel activation mechanism involving extracellular thioredoxin has been identified.
  • This mechanism links extracellular thioredoxin to cell function, with potential relevance to rheumatoid arthritis.
  • TRPC5-TRPC1 channels represent a new therapeutic target for inflammatory joint diseases.