Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compact biocompatible quantum dots functionalized for cellular imaging.

Wenhao Liu1, Mark Howarth, Andrew B Greytak

  • 1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.

Journal of the American Chemical Society
|January 8, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting the origin of electrochemical activity in the topological semimetal PtGa.

Chemical science·2026
Same author

Combining Ag(II) and Ag(I) Reactivity Enables Electrophotochemical Acyl Fluoride Installation on (Hetero)Arenes.

Journal of the American Chemical Society·2026
Same author

Visible-light-induced chlorine photoelimination from acridinium-phosphine gold(iii) complexes.

Chemical science·2026
Same author

Correction to "Near-Unity Triplet Quantum Yield in a Molecular Cofacial H-Dimer".

Journal of the American Chemical Society·2026
Same author

Structured Electrodes Induce Local pH as a Primary Determinant of CO<sub>2</sub> Reduction Selectivity.

Journal of the American Chemical Society·2026
Same author

Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking.

Nature communications·2026
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
See all related articles

We developed water-soluble quantum dots (QDs) with low cell binding and high stability. These QDs allow dual covalent and metal-affinity labeling for advanced biological imaging and sensing applications.

Area of Science:

  • Nanotechnology
  • Bioconjugation Chemistry
  • Cellular Imaging

Background:

  • Quantum dots (QDs) offer unique optical properties but often suffer from poor water solubility and nonspecific binding.
  • Developing stable, functionalizable nanomaterials is crucial for advanced biological applications.

Purpose of the Study:

  • To create water-soluble quantum dots (QDs) with versatile conjugation capabilities.
  • To enable simultaneous covalent and metal-affinity labeling for targeted cellular analysis.

Main Methods:

  • Synthesized water-soluble QDs functionalized with novel heterobifunctional ligands.
  • Employed carbodiimide coupling for covalent attachment of dyes and proteins (e.g., streptavidin).
  • Utilized metal-affinity chromatography for polyhistidine-tagged protein conjugation.

Related Experiment Videos

Main Results:

  • Achieved QDs with low nonspecific binding, small hydrodynamic diameter, and stability across a wide pH range.
  • Demonstrated successful covalent labeling via fluorescence resonance energy transfer (FRET) and high-affinity streptavidin attachment.
  • Showcased dual functionality enabling simultaneous covalent and metal-affinity conjugation on a single QD.

Conclusions:

  • The developed QDs are highly stable, water-soluble, and offer versatile dual conjugation strategies.
  • These QDs are suitable for tracking biomolecules like epidermal growth factor (EGF) on live cells.
  • The dual-emission properties pave the way for ratiometric sensing of cellular microenvironments.