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Updated: Jul 8, 2026
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A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
Published on: October 5, 2015
[Expression of lymphotactin in renal tuberculosis]
Xiong-bing Lu1, Hui-li Xie, Gao-de Zou
1Department of Urology, the Second Affiliated Hospital, Nanchang University, Nanchang 330006, China. luxiongbing123456@yahoo.com.cn
Summary
Lymphotactin (hLptn) is expressed in normal and tuberculous kidneys. However, the accumulation of CD4(+) and CD8(+) T cells in renal tuberculosis granulomas does not appear to be directly dependent on hLptn.
Area of Science:
- Immunology
- Nephrology
- Molecular Biology
Background:
- Renal tuberculosis involves complex immune responses within the kidney.
- Lymphotactin (hLptn) is a chemokine implicated in immune cell trafficking.
Purpose of the Study:
- To investigate hLptn expression in normal and tuberculous kidneys.
- To examine the colocalization of hLptn with CD4(+) and CD8(+) T cells in renal tuberculosis.
Main Methods:
- RT-PCR was used to amplify hLptn cDNA from kidney tissues.
- Immunohistochemical staining assessed hLptn, CD4, and CD8 expression.
- hLptn cDNA sequencing confirmed its identity.
Main Results:
- hLptn mRNA and protein were detected in both normal and tuberculous kidney cells, including glomeruli and tubules.
- CD4(+) and CD8(+) T cells were observed within granulomas in tuberculous kidneys.
- The sequence of cloned hLptn cDNA matched the GenBank reference.
Conclusions:
- hLptn is constitutively expressed in renal glomeruli and tubules in both healthy and diseased states.
- The infiltration of CD4(+) and CD8(+) T cells into granulomas in renal tuberculosis may occur independently of hLptn signaling.
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