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Antibiotic susceptibility testing at a screen-printed carbon electrode array.

Thomas S Mann1, Susan R Mikkelsen

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.

Analytical Chemistry
|January 10, 2008
PubMed
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This study presents a novel method for measuring antibiotic susceptibility in Escherichia coli using respiratory activity on screen-printed carbon electrodes. Electrochemical and chemical treatments effectively reduced antibiotic adsorption, enabling reliable susceptibility testing.

Area of Science:

  • Electrochemistry
  • Microbiology
  • Biosensing

Background:

  • Antibiotic resistance necessitates rapid and accurate susceptibility testing.
  • Traditional methods can be time-consuming.
  • Electrochemical biosensors offer potential for rapid detection.

Purpose of the Study:

  • To develop a respiratory activity-based method for antibiotic susceptibility testing of Escherichia coli.
  • To optimize screen-printed carbon electrodes to minimize antibiotic adsorption.
  • To evaluate the effectiveness of electrode modification techniques.

Main Methods:

  • Screen-printed carbon electrodes were modified using voltammetry in basic solution, poly-L-lysine, or chitosan.
  • Antibiotic adsorption onto electrodes was quantified.

Related Experiment Videos

  • Respiratory activity was measured via amperometry after incubation with antibiotics and an oxidative cocktail.
  • IC50 values for chloramphenicol were determined.

    • Main Results:

    • Electrochemical and chemical treatments effectively eliminated adsorption of 17 diverse antibiotics.
    • Response currents correlated with antibiotic concentration, indicating reduced respiratory activity.
    • IC50 values for chloramphenicol were consistent (2.0 ± 0.2 mM) across modification methods.
    • Results aligned with previous respiration-based studies but differed from growth-based methods.

    Conclusions:

    • Modified screen-printed carbon electrodes enable reliable respiratory activity-based antibiotic susceptibility testing.
    • Electrode modification is crucial for accurate measurements by preventing antibiotic adsorption.
    • This approach offers a promising alternative to traditional antibiotic susceptibility testing methods.