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Updated: Jul 8, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Enhanced observation of number fluctuations by use of structured light
Abstract:
The point statistics of light scattered from structured, incoherent illumination of a random and fluctuating population of identical scatterers are derived. Theoretical predictions for a two-beam illumination pattern in which the relative beam intensity is modulated are compared with computer simulations and experiments performed by illumination of a rotating mask of identical but random apertures. The use of structured light is found to enhance the size of fluctuations, and quantitative comparisons between theory and practical measurement are made. The structured-light technique introduces flexibility in fluctuation measurement, permitting optimal control of the dynamic range, and is relevant to the development of instrumentation for particle and flow analysis.
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