Related Experiment Video

Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Real-time observation of single-molecule fluorescence in microdroplet streams

M D Barnes, N Lermer, C Y Kung

    Optics Letters
    |August 15, 1997
    PubMed

    More Related Videos

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy08:39

    Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

    Published on: December 12, 2025

    Related Concept Videos

    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
    Super-resolution Fluorescence Microscopy01:37

    Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
    Total Internal Reflection Fluorescence Microscopy01:05

    Total Internal Reflection Fluorescence Microscopy

    Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.