Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nanoporesize affects complement activation.

Natalia Ferraz1, Bo Nilsson, Jaan Hong

  • 1Department of Physical and Analytical Chemistry, Division of Surface Biotechnology, Uppsala University, Uppsala 75123, Sweden.

Journal of Biomedical Materials Research. Part A
|January 11, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo evidence of effective complement modulation by TriFu in comparison to C5 inhibition and complement depletion.

Blood advances·2026
Same author

The Cellular Response Capacity as Diagnostic Head Start in Neutrophil Endotoxemia Sensing.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Reply to Comment on "A new principle to attenuate ischemia-reperfusion injury in kidney transplantation".

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Vulnerabilities, extreme weather and temporal tensions as experienced by construction workers in the Swedish construction sector.

PloS one·2026
Same author

Nuclear cell-free DNA on the loose: an early warning signal of ischemia-reperfusion injury in kidney transplantation.

Frontiers in immunology·2026
Same author

Native C3 is activated without proteolytic cleavage by transformation to C3(H<sub>2</sub>O) on phospholipid-scrambled cell membranes.

Frontiers in immunology·2026
Same journal

Granular Hydrogel Composites for Noninvasive Optical Biosensing.

Journal of biomedical materials research. Part A·2026
Same journal

Performance Evaluation of Highly Uniform Astragalus Polysaccharide/Silk Fibroin Microspheres Fabricated via Microfluidics as Biodegradable Fillers: Collagen Regeneration and Tissue Reaction.

Journal of biomedical materials research. Part A·2026
Same journal

Combining Supramolecular and Covalent Chemistry to Form Reinforced Fibrillar Network Hydrogels From Fibrinogen Derivatives.

Journal of biomedical materials research. Part A·2026
Same journal

Bioactive-Loaded Detachable Crosslinked Hyaluronic Acid Microneedles: Structural Validation and Clinical Anti-Wrinkle Efficacy.

Journal of biomedical materials research. Part A·2026
Same journal

Tunable Sol-Gel Transition in Poloxamer Blends for Injectable Osteoarticular Applications.

Journal of biomedical materials research. Part A·2026
Same journal

Development and Evaluation of Polyvinyl Alcohol/Carboxymethyl Chitosan Hydrocolloid Incorporating Cynodon dactylon Extract and Zinc Oxide Nanoparticle for Bedsore Healing in a Wistar Rat Model.

Journal of biomedical materials research. Part A·2026
See all related articles

Biomaterial nanotexture significantly impacts inflammatory responses. A slight increase in nanopore size (20 to 200 nm) on alumina membranes greatly affects the complement system, influencing protein adsorption and activation.

Area of Science:

  • Biomaterials Science
  • Immunology
  • Surface Chemistry

Background:

  • Biomaterial surface topography is crucial for biological interactions.
  • Nanoporous alumina is a promising material for controlling cellular and protein events.
  • Understanding inflammatory responses to biomaterials is key for implant development.

Purpose of the Study:

  • To investigate the effect of nanopore size on biomaterial-induced inflammatory responses.
  • To evaluate the impact of alumina nanotexture on the complement system in a whole blood model.
  • To determine how different pore sizes influence protein adsorption and complement activation.

Main Methods:

  • Utilized nanoporous alumina membranes with pore sizes of 20 nm and 200 nm.
  • Conducted in vitro whole blood studies assessing complement activation.

Related Experiment Videos

  • Analyzed fluid phase complement components (C3a, sC5b-9) and surface-adsorbed proteins (IgG, IgM, C1q, C3).

    • Main Results:

    • A 200 nm pore size significantly increased complement activation compared to 20 nm.
    • Higher levels of soluble complement components (C3a, sC5b-9) were observed with 200 nm pores.
    • Increased adsorption of IgG, IgM, C1q, and C3 was found on the 200 nm alumina membranes.

    Conclusions:

    • Alumina nanopore size critically influences complement activation and protein adsorption.
    • Material topography dictates protein patterns, affecting the inflammatory response.
    • Nanoporous alumina offers tunable properties for controlling biomaterial-host interactions.