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Updated: Jul 8, 2026

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
New values of molecular extinction coefficient and specific rotation for cyanobacterial toxin cylindrospermopsin
Tomoharu Sano1, Sachiko Kikuchi, Takuya Kubo
1National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
Abstract:
The molecular extinction coefficient epsilon of cylindrospermopsin (CYN) purified by the anion exchange and the normal-phase HPLC procedures was determined to be 9800 at 262 nm. This epsilon is significantly higher than those (epsilon, 5800-6250) reported previously. In order to determine CYN concentrations in solutions using UV absorption, the epsilon-value of CYN should be corrected from 5800 to 9800. Further, the [alpha](D) value of CYN should be corrected from +12.5 degrees to +17.0 degrees .

