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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 8, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Antibody microarray analysis of directly labelled complex proteomes.

Christer Wingren1, Carl Ak Borrebaeck

  • 1Department of Immunotechnology, Lund University, BMC D13, SE-221 84 Lund, Sweden. christer.wingren@immun.lth.se

Current Opinion in Biotechnology
|January 12, 2008
PubMed
Summary

Antibody microarray technology now targets complex proteomes, focusing on sample and data handling. Direct biotin labeling and bioinformatic standards improve analysis, though SOPs are still needed.

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Last Updated: Jul 8, 2026

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13:21

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Published on: May 4, 2012

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Area of Science:

  • Biotechnology
  • Proteomics
  • Bioinformatics

Background:

  • Antibody microarray technology has advanced significantly, moving from concept to high-performance platforms for complex proteome analysis.
  • Key challenges in antibody microarray development involve sample and data handling.
  • Direct labeling protocols and bioinformatic standards are emerging.

Purpose of the Study:

  • To highlight advancements in antibody microarray technology, particularly in sample and data handling.
  • To discuss the development of robust protocols for direct proteome labeling and analysis.
  • To introduce emerging bioinformatic standards for antibody microarray data.

Main Methods:

  • Development of robust protocols for direct labeling of whole proteomes.
  • Utilization of sensitive fluorescent-based sensing.
  • Adaptation of methods from DNA microarray analysis for bioinformatic standards.

Main Results:

  • Established high-performing antibody microarray platforms capable of targeting non-fractionated complex proteomes.
  • Designed robust protocols for direct labeling of whole proteomes.
  • Developed initial bioinformatic standards for antibody microarray data analysis, with biotin tagging as a preferred approach.

Conclusions:

  • Antibody microarray technology is a powerful tool for proteome analysis.
  • Direct labeling and improved data handling are crucial for advancing the technology.
  • Further standardization of operating procedures is necessary for widespread adoption.