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Updated: Jul 8, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Instrumentation for measuring fluorescence cross sections from airborne microsized particles
A Manninen1, M Putkiranta, A Rostedt
1Department of Physics, Tampere University of Technology, Tampere, Finland. albert.manninen@tut.fi
Abstract:
An experimental instrument for measuring a laser-induced fluorescence spectrum from a single aerosol particle is described. As a demonstration of instrument capabilities, the results of monodisperse 4.7 microm sodium chloride particles doped with fluorescent riboflavin, produced with an inkjet aerosol generator, are presented. The fluorescence of the aerosol particles is excited in the wide range from 210 to 419 nm using a pulsed, tunable optical parametric oscillator laser. The maximum of the fluorescence emission of separately measured particles is detected at 560 nm. The dependence of the fluorescence on the excitation wavelength is studied and fluorescence cross sections are estimated. Agreement between the measured fluorescence data and the literature data for riboflavin is observed.
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