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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Overview Of Cell Separation And Isolation

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Updated: Jul 8, 2026

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Published on: June 4, 2015

Blood cell interactions and segregation in flow.

Lance L Munn¹, Michael M Dupin

¹E.L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 100 Blossom St., COX 7, Boston, MA 02114, USA. munn@steele.mgh.harvard.edu

Annals of Biomedical Engineering

|January 12, 2008

Summary

Researchers explore blood flow dynamics, revealing how blood cells interact with each other and vessel walls. This study builds on a century of research into the non-Newtonian nature of blood for improved physiological understanding.

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Area of Science:

Biophysics
Hemodynamics
Cellular Biology

Background:

Blood flow exhibits complex, non-Newtonian properties due to cellular behavior.
Cell deformability and reversible aggregation are key factors in blood's unique fluid dynamics.
Pioneering work, including Harry Goldsmith's, advanced visualization of cellular blood flow.

Purpose of the Study:

To further elucidate the intricate interactions between blood cells.
To enhance understanding of blood cell interactions with the blood vessel wall.
To build upon historical research in blood flow dynamics.

Main Methods:

Utilizing novel experimental and computational approaches.
Applying advanced visualization and quantification techniques for single-cell analysis.
Continuing the tradition of innovative research in hemorheology.

Main Results:

Blood cells demonstrate preferential migration to the flow axis.
Distinct segregation patterns observed between red and white blood cells during flow.
Cellular properties significantly contribute to blood's non-Newtonian characteristics.

Conclusions:

Understanding cellular interactions is crucial for comprehending blood's physiological functions.
Continued research is vital for advancing knowledge of blood flow and its implications.
This work contributes to the ongoing effort to unravel blood's complex flow behavior.