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Updated: Jul 8, 2026

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
08:37

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Published on: June 1, 2017

Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of

Etienne H Meyer1, Nicolas L Taylor, A Harvey Millar

  • 1ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia.

Journal of Proteome Research
|January 15, 2008
PubMed

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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
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