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Updated: Jul 8, 2026
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Published on: September 28, 2013
Heat-shock response in Arabidopsis thaliana explored by multiplexed quantitative proteomics using differential
Magnus Palmblad1, Davinia J Mills, Laurence V Bindschedler
1The BioCentre, The University of Reading, Whiteknights, Post Office Box 221, Reading RG6 6AS, United Kingdom. n.m.palmblad@lumc.nl
Abstract:
We have developed a general method for multiplexed quantitative proteomics using differential metabolic stable isotope labeling and mass spectrometry. The method was successfully used to study the dynamics of heat-shock response in Arabidopsis thaliana. A number of known heat-shock proteins were confirmed, and some proteins not previously associated with heat shock were discovered. The method is applicable in stable isotope labeling and allows for high degrees of multiplexing.
