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Updated: Jul 8, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
High-throughput log P determination by ultraperformance liquid chromatography: a convenient tool for medicinal
Yveline Henchoz1, Davy Guillarme, Serge Rudaz
1Unité de Pharmacochimie, School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Quai E-Ansermet 30, Geneva, Switzerland.
Abstract:
Accurate determinations of lipophilicity indices benefit from recent advances in chromatographic sciences such as the launch of ultraperformance liquid chromatography (UPLC). The fast strategy presented here emerges as a powerful method suitable for high-throughput log P measurements of therapeutic compounds in isocratic and gradient modes. Because UPLC columns are highly stable in basic pH conditions, this approach allows a direct lipophilicity estimation of basic compounds in their neutral forms.
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