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Glycation of lysozyme in a restricted water environment
H Wu1, T G Hartman, S Govindarajan
1Department of Food Science, Cook College, Rutgers, The State University of New Jersey.
Abstract:
Fast atom bombardment mass spectrometry (FAB) was used to determine the glycation sites of lysozyme in a restricted water environment. A 30-day incubation at 25 degrees C, and 65% relative humidity (R.H.) resulted in glycation at lysine-1 while a much shorter (3-day) incubation at 50 degrees C and 65% R.H. resulted in diglycation at lysine-1 as well as glycation at lysine-13 and lysine-33.