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Related Experiment Videos

Complex target SELEX.

Steven M Shamah1, Judith M Healy, Sharon T Cload

  • 1Archemix Corporation, 300 Third Street, Cambridge, Massachusetts 02142, USA.

Accounts of Chemical Research
|January 16, 2008
PubMed
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Aptamer technology, using systematic evolution of ligands by exponential enrichment (SELEX), now targets complex cell surface proteins. This enables discovery of novel aptamers for diagnostics and therapeutics against challenging targets.

Area of Science:

  • Biotechnology and Molecular Biology
  • Nucleic Acid Therapeutics
  • Drug Discovery and Development

Background:

  • Aptamers are structured oligonucleotides with high affinity and specificity for molecular targets.
  • Traditional SELEX (systematic evolution of ligands by exponential enrichment) often requires purified soluble proteins.
  • Cell surface proteins crucial for proper folding or function are difficult targets for conventional SELEX.

Purpose of the Study:

  • To review recent advancements in SELEX for generating aptamers against cell surface targets.
  • To highlight the utility of SELEX using complex biological samples like membrane preparations and whole cells.
  • To demonstrate the potential of aptamers for therapeutic and diagnostic applications against previously intractable targets.

Main Methods:

Related Experiment Videos

  • Utilizing membrane preparations or whole cells as targets for SELEX.
  • Applying SELEX to diverse biological scaffolds including cell fragments, parasites, bacteria, viruses, and human cell lines.
  • Isolating aptamers against cell-surface proteins without prior detailed target knowledge.

Main Results:

  • Successful generation of aptamers against cell surface targets using complex SELEX approaches.
  • Demonstrated efficacy of SELEX with various biological scaffolds, including stem cells and tumor lines.
  • Identification of aptamers targeting cell differentiation markers and pathogenic determinants.

Conclusions:

  • Advanced SELEX techniques enable aptamer discovery against challenging cell surface targets.
  • This approach broadens the therapeutic and diagnostic potential of aptamers.
  • Aptamers generated against complex targets show promise for diverse applications in medicine and research.