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Updated: Jul 8, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Scanning and surface alignment considerations in chemical imaging with desorption electrospray mass spectrometry
Vilmos Kertesz1, Gary J Van Berkel
1Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov
Abstract:
The effects of surface scanning mode (raster vs unidirectional scanning) and the constancy of spray tip-to-surface and atmospheric sampling interface capillary-to-surface distances on chemical image quality using desorption electrospray ionization mass spectrometry were investigated. Unidirectional scanning was found to provide a spatially and a quantitatively more precise chemical image of the surface as compared to raster scanning. Maintaining constant spray tip-to-surface and atmospheric sampling interface capillary-to-surface distances during an imaging experiment was found to also be critical. An automation process was implemented using a custom image analysis software (HandsFree Surface Analysis) to keep these distances constant during the surface sampling experiment. Improved chemical image quality afforded through this software control was illustrated by imaging printed objects on normal copy paper.
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