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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Radiation pattern of fluorescence from molecules embedded in small particles: general case.
1Clarkson College of Technology, Physics Department, Potsdam, New York 13676, USA.
Applied Optics
|January 1, 1983
Summary
This study introduces a new method to analyze fluorescent emission, accounting for molecular reorientation. The model accurately predicts experimental data for dye molecules in microspheres.
Area of Science:
- Molecular spectroscopy
- Photophysics
- Materials science
Background:
- Anisotropic molecules in dielectric particles exhibit complex fluorescent emission patterns.
- Previous models often simplified or ignored the reorientation angle between absorption and emission moments.
- Understanding these angular distributions is crucial for applications in sensing and imaging.
Purpose of the Study:
- To develop and validate a new computational method for analyzing fluorescent emission.
- To incorporate the physical consideration of a non-zero reorientation angle between absorption and emission moments.
- To accurately model the angular distribution of fluorescence from anisotropic molecules in microspheres.
Main Methods:
- A novel calculation method was developed to analyze angular emission distributions.
- The method explicitly accounts for a non-zero reorientation angle between absorption and emission transition moments.
- Computational results were compared against experimental data for fluorescent dye-doped microspheres.
Main Results:
- The developed method shows good quantitative agreement with experimental data.
- The fitting process effectively determines the effective reorientation angle between transition moments.
- The model provides a more physically realistic description of fluorescent emission.
Conclusions:
- The new method offers a robust framework for analyzing fluorescent emission from anisotropic molecules in particles.
- The inclusion of a reorientation angle significantly improves the accuracy of emission distribution predictions.
- This work facilitates more precise characterization of fluorescent materials and systems.
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