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Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Published on: August 20, 2017

Putative embryonic stem cell lines from pig embryos.

Irena Vackova1, Andrea Ungrova, Federica Lopes

  • 1Institute of Animal Science, Praha Uhrineves, Prague, Czech Republic. ivackova@seznam.cz

The Journal of Reproduction and Development
|January 17, 2008
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Summary

Establishing porcine embryonic stem cell (ES cell) lines is crucial for pre-clinical research. This review explores the challenges and recent advances in deriving these valuable cells in pigs.

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Area of Science:

  • Stem cell biology
  • Developmental biology
  • Comparative embryology

Background:

  • Embryonic stem cells (ES cells) are pluripotent cells from early embryos, capable of differentiating into all cell types.
  • ES cells can be genetically modified and used to create chimeras, but this has been limited to mice.
  • Pigs are ideal models for pre-clinical research, necessitating the development of porcine ES cell lines.

Purpose of the Study:

  • To review recent advances in deriving porcine ES cell lines.
  • To discuss obstacles hindering the successful derivation of these cells in pigs.
  • To highlight the importance of porcine ES cells for pre-clinical applications.

Main Methods:

  • Review of current literature on ES cell derivation in various species.
  • Analysis of factors influencing ES cell pluripotency and differentiation.
  • Comparative study of ES cell derivation techniques and their applicability to pigs.

Main Results:

  • Despite significant research, convincing porcine ES cell lines have not yet been established.
  • Several technical and biological hurdles impede porcine ES cell derivation.
  • Understanding these obstacles is key to future success.

Conclusions:

  • Deriving porcine ES cell lines remains a significant challenge in stem cell research.
  • Overcoming these challenges is essential for advancing pre-clinical studies using pigs.
  • Further research into porcine developmental biology and stem cell culture is required.