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Related Experiment Videos

Covalent virus layer for mass-based biosensing.

Li-Mei C Yang1, Juan E Diaz, Theresa M McIntire

  • 1Department of Chemistry, University of California, Irvine, CA 92697-2025, USA.

Analytical Chemistry
|January 18, 2008
PubMed
Summary
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This study developed a covalent virus surface (CVS) using M13 phage for sensitive antibody detection. The robust CVS demonstrates high selectivity and sensitivity for mass-based biodetection applications.

Area of Science:

  • Biomaterials Science
  • Biosensor Technology
  • Nanotechnology

Background:

  • Developing sensitive and selective biodetection methods is crucial for diagnostics.
  • Immobilization of biological entities onto sensor surfaces presents challenges in stability and density.
  • Phage display technology offers a platform for creating ordered, high-density biological surfaces.

Purpose of the Study:

  • To create a stable and dense covalent virus surface (CVS) for mass-based biodetection.
  • To evaluate the performance of the CVS for detecting antibodies using quartz crystal microbalance (QCM).
  • To determine the binding capacity and selectivity of the immobilized M13 phage.

Main Methods:

  • Covalent attachment of M13 virus particles to a gold-coated QCM using a self-assembled monolayer.

Related Experiment Videos

  • Formation of a phage multilayer with high surface coverage.
  • Mass-based detection of antibodies via flow injection analysis and QCM measurements.
  • Regeneration of the sensor surface using HCl washing.

    • Main Results:

    • The CVS exhibited a mass increase upon binding of a specific antibody (p-Ab).
    • A linear calibration plot for p-Ab binding was established with high sensitivity (0.018 microg/(cm2 nM)) and a low limit of detection (7 nM).
    • The CVS demonstrated selectivity, with no significant mass change upon exposure to a non-binding antibody (n-Ab).
    • Approximately 140 binding sites per M13 phage particle were quantified.

    Conclusions:

    • The covalent virus layer is a robust, selective, and sensitive platform for mass-based biodetection.
    • This method enables quantitative analysis of antibody binding.
    • The developed CVS shows potential for various biosensing applications.