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Updated: Jul 8, 2026

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
Published on: February 26, 2018
Wapl localization on the synaptonemal complex, a meiosis-specific proteinaceous structure that binds homologous
J Zhang1, H Håkansson, M Kuroda
1Unit of Environmental Health Risk Assessment, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Abstract:
The wings apart-like (Wapl) protein is required to hold sister chromatids together in mitotic heterochromatin in Drosophila melanogaster. It is localized on the synaptonemal complex (SC), a meiosis-specific structure connecting one pair of sister chromatids to the homologous pair in mouse pachytene spermatocytes. The human Wapl is a cohesin-binding protein that facilitates cohesin's timely release from chromosome arms during prophase. The objective of the present study was to determine the subcellular localization of the mouse Wapl on female meiotic chromosomes at pachynema. The pachytene oocytes were isolated from foetal ovaries at 18.5 dpc and double immunostained with anti-synaptonemal complex protein 2 (SYCP2) and anti-Wapl. In the pachytene oocytes examined, mouse Wapl was colocalized with SYCP2 on the SC. Our results further implicated that Wapl might play a crucial role in meiotic chromosome remodelling at early meiosis.
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