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A simple technique for large scale in vitro culture of Plasmodium falciparum
P Chavalitshewinkoon1, P Wilairat
1Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
The Southeast Asian Journal of Tropical Medicine and Public Health
|December 1, 1991
Abstract:
The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.