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Updated: Jul 8, 2026
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Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
A maximal isokinetic pedalling exercise for EMG normalization in cycling.
Eneko Fernández-Peña1, Francesco Lucertini, Massimiliano Ditroilo
1Istituto di Ricerca sull'Attività Motoria, Università degli Studi di Urbino Carlo Bo, Via I Maggetti, 26/2, 61029 Urbino, Italy.
Summary
This study introduces a new maximal isokinetic protocol (MIP) for reliable electromyography (EMG) amplitude normalization in cycling. Three trials of MIP are sufficient for accurate EMG normalization in cyclists.
Area of Science:
- Sports Science
- Biomechanics
- Exercise Physiology
Background:
- Electromyography (EMG) normalization is crucial for comparing muscle activity.
- Isometric maximal voluntary contraction (iMVC) is commonly used but has limitations.
Purpose of the Study:
- To propose and assess the reliability of a novel EMG amplitude normalization method for cycling.
- To establish a cycling-specific protocol for EMG normalization.
Main Methods:
- Twenty-three cyclists performed maximal isokinetic protocols (MIP) and sub-maximal trials on a cycle ergometer.
- EMG activity of four lower limb muscles was recorded during protocols.
- Reliability was assessed through coefficient of variation (CV) and reproducibility analysis.
Main Results:
- MIP demonstrated steady power output (CV=2.19) and EMG activity (CV=4.46-8.70).
- Maximal EMG values were consistently reached by the 4th trial.
- MIP showed good logical validity and within-subject reproducibility, suggesting three trials are adequate.
Conclusions:
- The maximal isokinetic protocol (MIP) is a reliable and cycling-specific method for EMG amplitude normalization.
- Three MIP trials are sufficient for accurate EMG normalization in cycling.
- This method overcomes limitations associated with iMVC in cycling research.
