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Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: Jul 8, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

The MCM helicase: linking checkpoints to the replication fork.

Susan L Forsburg1

  • 1Molecular and Computational Biology Section, University of Southern California, 1050 Childs Way MCB 201B, Los Angeles, CA 90089-2910, USA. forsburg@usc.edu

Biochemical Society Transactions
|January 23, 2008
PubMed
Summary
This summary is machine-generated.

The minichromosome maintenance (MCM) complex, a key DNA helicase, is vital for replication fork stability and genome integrity. MCM proteins interact with checkpoint and repair pathways, acting as crucial effectors in maintaining genomic stability.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Last Updated: Jul 8, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
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Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • The minichromosome maintenance (MCM) complex is an essential DNA helicase.
  • The MCM complex plays a critical role in DNA replication.
  • Emerging evidence suggests MCM's involvement in replication fork integrity and checkpoint regulation.

Purpose of the Study:

  • To elucidate the role of the MCM complex in maintaining replication fork integrity.
  • To investigate the MCM complex as a potential target of the replication checkpoint.
  • To understand the interactions of MCM proteins with other cellular machinery involved in DNA repair and genome maintenance.

Main Methods:

  • Analysis of MCM complex interactions with checkpoint kinases.
  • Investigation of MCM protein involvement in DNA repair pathways.
  • Studies on the functional significance of MCMs in maintaining replication fork stability.

Main Results:

  • The MCM helicase is crucial for maintaining replication fork integrity.
  • MCM proteins are implicated as targets of the replication checkpoint.
  • Interactions between MCMs, checkpoint kinases, and repair proteins highlight MCMs' role in genome stability.

Conclusions:

  • The MCM complex is a critical effector of replication fork stability.
  • MCM proteins are integral to the cell's response to replication stress.
  • The MCM complex plays a significant role in maintaining overall genome integrity.