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Updated: Jul 8, 2026

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Published on: March 3, 2014
Botox: shaken, not stirred
1Department of Ophthalmology, Kresge Eye Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Purpose:
To determine whether the effect of botulinum toxin type A is maintained and has the same duration when it is reconstituted vigorously.
Methods:
A prospective, double-blinded, randomized study was performed on 7 consecutive patients who underwent botulinum toxin type A injections to the forehead by one oculoplastic surgeon. Half of each patient's forehead was injected with botulinum toxin type A that had been gently reconstituted according to the package insert and the other half of the forehead was injected with botulinum toxin type A that had been reconstituted vigorously. Eyebrow excursion was measured in millimeters before injection, 1 week after injection, and every month after injection up to a total of 6 months.
Results:
Seven consecutive patients with an average age of 39.9 +/- 2.8 years were evaluated. There was no statistically significant difference in eyebrow excursion between the side of the forehead that had been injected with gently reconstituted botulinum toxin type A and the side that had been injected with vigorously reconstituted botulinum toxin type A at every visit.
Conclusion:
The effect of botulinum toxin type A is maintained and has the same duration when it is reconstituted vigorously compared with when it is reconstituted gently.
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