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Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
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A LuxP-based fluorescent sensor for bacterial autoinducer II.

Jinge Zhu1, Dehua Pei

  • 1Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.

ACS Chemical Biology
|January 25, 2008
PubMed
Summary

Researchers developed novel protein sensors for quantifying autoinducer 2 (AI-2), a key molecule in bacterial communication. This new method offers a more accurate way to measure AI-2 levels in various bacterial environments.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Autoinducer 2 (AI-2) facilitates interspecies bacterial communication.
  • Current AI-2 detection methods, like the Vibrio harveyi bioassay, are not quantitative and are sensitive to experimental conditions.

Purpose of the Study:

  • To develop a quantitative method for AI-2 detection.
  • To create novel protein-based sensors for AI-2.

Main Methods:

  • Engineered AI-2 receptor proteins (LuxP and LsrB) by incorporating environmentally sensitive fluorescent dyes.
  • Developed a fluorescence-based assay utilizing these modified proteins.

Main Results:

  • The developed protein sensors specifically bind to AI-2.
  • Sensors exhibit dose-dependent changes in fluorescence yield upon AI-2 binding.
  • The assay successfully monitored AI-2 enzymatic synthesis in real-time.
  • Extracellular and intracellular AI-2 concentrations were determined in bacterial cultures.

Conclusions:

  • The novel protein sensors provide a quantitative and reliable method for AI-2 detection.
  • This advancement enables precise monitoring of bacterial communication signals.