Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

3.0K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
3.0K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

1.3K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A cocktail of SARS-CoV-2 S stem helix domain and receptor binding domain human monoclonal antibodies prevents the emergence of viral escape mutants.

Microbiology spectrum·2026
Same author

Establishment of a Neonatal Natural Transmission Model for CMV Vaccine Development.

The Journal of infectious diseases·2026
Same author

A bireporter recombinant SARS-CoV-2 Omicron BA.5 for <i>in vitro</i> and <i>in vivo</i> studies.

bioRxiv : the preprint server for biology·2026
Same author

The structure of <i>Streptococcus gordonii</i> surface protein SspB in complex with TEV peptide provides clues to oral streptococcal adherence to salivary agglutinin.

Infection and immunity·2026
Same author

A cocktail of SARS-CoV-2 spike stem helix domain and receptor binding domain human monoclonal antibodies prevent the emerge of viral escape mutants.

bioRxiv : the preprint server for biology·2025
Same author

Streptococcus mutans Collagen-Binding Protein Cnm Is a Multifunctional Adhesin: A Structural Investigation.

Molecular oral microbiology·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Oct 2, 2025

High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

2.5K

Protein crystallization.

Champion Deivanayagam1, William J Cook, Mark R Walter

  • 1Center for Biophysical Sciences and Engineering, Department of Vision Sciences, University of Alabama at Birmingham, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 26, 2008
PubMed
Summary
This summary is machine-generated.

X-ray crystallography determines biological macromolecule structures. Advances enable high-throughput structure determination, but protein production and crystallization remain key challenges, with ongoing robotic automation efforts.

More Related Videos

From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

13.6K
Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

64.0K

Related Experiment Videos

Last Updated: Oct 2, 2025

High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

2.5K
From Constructs to Crystals &#8211; Towards Structure Determination of &#946;-barrel Outer Membrane Proteins
09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

13.6K
Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

64.0K

Area of Science:

  • Structural biology
  • Biochemistry
  • Biophysics

Background:

  • X-ray crystallography is a vital technique for elucidating the 3D structures of biological macromolecules and their complexes.
  • Significant advancements in data collection, structure solution, and refinement are propelling the field towards high-throughput, genomic-scale structure determination.

Purpose of the Study:

  • To provide essential information on standard protein production and crystallization methods.
  • To highlight ongoing efforts in applying high-throughput robotics to accelerate these processes.

Main Methods:

  • Standardized protocols for protein production and crystallization.
  • Implementation of high-throughput robotic systems for automation.

Main Results:

  • The chapter details established methods for protein production and crystallization.
  • It discusses the integration of robotics to address bottlenecks in these areas.

Conclusions:

  • Protein production and crystallization are critical bottlenecks in high-throughput structure determination.
  • Robotic automation shows promise for increasing the speed and efficiency of macromolecular structure determination.