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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
Published on: March 15, 2011
Processing protocols for high quality glass-based microarrays: applications in DNA, peptide, antibody, and
Jens Sobek1, Catharine Aquino, Ralph Schlapbach
1Functional Genomics Center Zurich, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2008
Summary
Standardized microarray protocols, optimized for surface chemistry, provide a foundation for successful experiments. This guide offers processing parameters for DNA, peptide, antibody, and carbohydrate microarrays to achieve high-quality results.
Area of Science:
- Biotechnology
- Molecular Biology
- Analytical Chemistry
Background:
- Microarray technology relies on specific surface chemistry and optimized processing for reliable results.
- Previous work discussed quality considerations and surface chemistry selection for microarrays.
- A defined workflow aids in selecting slide surface architectures and optimizing processing parameters.
Purpose of the Study:
- To outline processing parameters for various microarray types.
- To serve as a starting point for establishing robust microarray experiments.
- To enable direct generation of high-quality results for popular microarray applications.
Main Methods:
- Selection of appropriate surface chemistry.
- Utilization of a defined workflow for optimization.
- Application of outlined processing parameters for DNA, peptide, antibody, and carbohydrate microarrays.
Main Results:
- Established processing parameters for diverse microarray types.
- Demonstrated a pathway for iterative experimental establishment.
- Facilitated direct generation of high-quality microarray data.
Conclusions:
- Optimized surface chemistry and processing workflows are crucial for microarray success.
- The provided parameters offer a reliable starting point for various microarray applications.
- This approach streamlines the generation of high-quality microarray data.
