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Updated: Jul 8, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

A microarray-based method to profile global microRNA expression in human and mouse.

Ranjan J Perera¹

¹Keck Graduate Institute, Claremont, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2008

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression. This study details a method to profile miRNA expression, revealing specific miRNAs crucial for adipocyte differentiation and kidney function.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression in eukaryotes.
Over 200 distinct miRNA genes have been identified across various organisms.
miRNAs are involved in diverse developmental and cellular processes through translation repression or RNA interference.

Purpose of the Study:

To present a detailed protocol for profiling miRNA expression in eukaryotic tissues, organs, and cell lines.
To identify miRNAs preferentially expressed in human primary adipocytes.
To investigate the role of specific miRNAs in adipocyte differentiation and kidney physiological maintenance.

Main Methods:

Utilized a microarray-based method for robust miRNA expression profiling in mammalian cells and tissues.
Identified differentially expressed miRNAs in human primary adipocytes.
Investigated the functional impact of miRNA knockdown on adipocyte differentiation.
Analyzed conserved upstream regulatory elements (Ets 1) in kidney-specific miRNAs.

Main Results:

A group of miRNAs were found to be preferentially expressed in human primary adipocytes.
Knockdown of miRNA 143 was observed to reverse the adipocyte differentiation process.
Kidney-specific miRNAs were identified, potentially linked to kidney physiological maintenance due to conserved upstream elements.

Conclusions:

The developed microarray method provides a robust approach for global miRNA profiling.
Specific miRNAs play critical roles in adipocyte differentiation.
Conserved regulatory elements suggest important functions for kidney-specific miRNAs in maintaining kidney physiology.