Related Experiment Video

Updated: Jul 1, 2025

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

16.3K

A therapeutically targetable positive feedback loop between lnc-HLX-2-7, HLX, and MYC that promotes group 3

Keisuke Katsushima1, Kandarp Joshi1, Menglang Yuan1

  • 1Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, School of Medicine, Johns Hopkins University, 1650 Orleans St., Baltimore, MD 21231, USA; Johns Hopkins All Children's Hospital, 600 5th St. South, St. Petersburg, FL 33701, USA.

Cell Reports
|March 9, 2024
PubMed

Insights

A newly identified long non-coding RNA, lnc-HLX-2-7, drives group 3 medulloblastoma (G3 MB) progression. Targeting this RNA with nanoparticles significantly inhibits tumor growth and enhances survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • Group 3 medulloblastoma (G3 MB) is an aggressive pediatric brain tumor with limited therapeutic options.

Purpose of the Study:

  • To investigate the role of lnc-HLX-2-7 in G3 MB pathogenesis.
  • To evaluate lnc-HLX-2-7 as a potential therapeutic target for G3 MB.

Main Methods:

  • RNA sequencing and chromatin immunoprecipitation were used to identify and characterize the lnc-HLX-2-7 regulatory pathway.
  • In vivo studies utilized an intracranial G3 MB xenograft mouse model treated with cerium-oxide-nanoparticle-coated antisense oligonucleotides (CNP-lnc-HLX-2-7).

Main Results:

  • lnc-HLX-2-7 was found to activate the oncogene HLX by recruiting enhancer elements.
  • HLX was shown to activate key oncogenes including MYC, LIN9, TBX2, and HOXM1.
  • CNP-lnc-HLX-2-7 treatment reduced tumor growth by 40%-50% in a mouse model.
  • Combination therapy with CNP-lnc-HLX-2-7 and cisplatin significantly improved survival.

Conclusions:

  • The lnc-HLX-2-7-HLX-MYC axis is a critical regulator of G3 MB progression.
  • lnc-HLX-2-7 represents a promising therapeutic target for G3 MB.
Keywords:
CP: CancerCP: NeuroscienceeRNAlnc-HLX-2-7medulloblastomananoparticlestherapeutics

More Related Videos

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice05:10

Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice

Published on: October 3, 2010

12.2K
Zebrafish Model of Neuroblastoma Metastasis05:20

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

2.8K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.6K