Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 8, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

ArrayPlex SA: a turn-key automated gene expression target preparation system.

Handy Yowanto1

  • 1Beckman Coulter, Inc., Fullerton, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a gene expression biomarker to identify operationally tolerant liver transplant recipients: the LITMUS trial.

Clinical and experimental immunology·2022
Same author

Multicapillary gel electrophoresis based analysis of genetic variants in the WFS1 gene.

Electrophoresis·2016
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Automated target preparation for gene expression analysis significantly reduces hands-on time and errors. This streamlined process on the ArrayPlex SA system enables high-throughput sample processing for accurate gene expression studies.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Manual target preparation for gene expression analysis is time-consuming, labor-intensive, and prone to errors.
  • Current methods divert scientists from critical research activities.

Purpose of the Study:

  • To develop and validate automated target preparation methods for gene expression analysis.
  • To streamline the workflow, reduce processing time, and minimize reagent costs.

Main Methods:

  • Development of automated target preparation methods on the ArrayPlex SA system, incorporating cDNA synthesis, in vitro transcription, and fragmentation.
  • Processing of up to 96 samples in under 36 hours.
  • Comparison of automated method with manual processing using Affymetrix GeneChip arrays.

More Related Videos

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Related Experiment Videos

Last Updated: Jul 8, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Main Results:

  • The ArrayPlex SA system successfully automated target preparation, including cDNA synthesis, in vitro transcription, and fragmentation.
  • The automated process allowed for high-throughput processing of up to 96 samples in less than 36 hours.
  • Nested analysis of variance identified sources of variability between automated and manual methods.

Conclusions:

  • Automated target preparation on the ArrayPlex SA system offers a streamlined, efficient, and accurate alternative to manual methods.
  • This automation enhances throughput and reduces hands-on time for gene expression analysis.
  • The developed methods provide a robust platform for consolidating runs, optimizing instrument time, and minimizing reagent costs.