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Updated: Jul 7, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
A simple and fast method for quantification of ertapenem using meropenem as internal standard in human plasma in a
Antonio D'Avolio1, Lorena Baietto, Francesco Giuseppe De Rosa
1Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera, Turin, Italy. antodav@inwind.it
Abstract:
A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 microg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.
