Visualization and experimental analysis of blood vessel formation using transgenic zebrafish

The mechanisms of blood vessel formation have become a subject of enormous scientific and clinical interest. However, it is difficult to visualize the developing vasculature in most living animals due to the ubiquitous and deep localization of vessels within other tissues. The establishment of vascular-specific transgenic zebrafish with fluorescently "tagged" blood vessels has facilitated high-resolution imaging studies of developing blood and lymphatic vessels in vivo. Use of these transgenic lines for genetic and chemical screening, experimental manipulations, and time-lapse imaging has extended our knowledge of how complex networks of vessels assemble in vivo.