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Updated: Jul 7, 2026
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Preparation and Fractionation of Xenopus laevis Egg Extracts
Published on: August 27, 2008
Preparation and use of interphase Xenopus egg extracts
Current Protocols in Cell Biology
|January 30, 2008
Abstract:
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
